SubtiBank SubtiBank
lnrL [2019-04-24 13:27:16]
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lnrL [2019-04-24 13:27:16]

similar to [SW|ABC transporter] (ATP-binding protein), involved in resistance to linearmycin
locus
BSU08310
pI
6.50
mw
33.95 kDa
protein length
311 aa Sequence Blast
gene length
936 bp Sequence Blast
function
resistance to linearmycin
product
[SW|ABC transporter] (ATP-binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.1|Efflux of antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    905,816 906,751

    The protein

    Protein family

  • [SW|ABC transporter] domain (according to Swiss-Prot)

    • Structure

  • [PDB|4YER] (from Thermotoga maritima, 39% identity)

    • [SW|Localization]

  • membrane associated (via [protein|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|LnrM]-[protein|79BAF2329209C81E8D165F131F9DAADB4A81448A|LnrN]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]-[gene|07858BFC72EB0B6AF615C8D4C8BCC162D8C3E7D2|lnrM]-[gene|79BAF2329209C81E8D165F131F9DAADB4A81448A|lnrN]
    description
    [Pubmed|26647299]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]: activation, [Pubmed|26647299], in [regulon|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK regulon]

    • regulation

  • expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
  • induced in the presence of linearmycin and other polyenes ([protein|387EF370CE24F7A3C20789A57329A02EBED46F53|LnrK]) [pubmed|32973732,28461449]
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    Biological materials

    Mutant

  • MGNA-C354 (yfiL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2352 NBRP B. subtilis, Japan]
  • BKE08310 ([gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCGTCTCACTCCTTTT, downstream forward: _UP4_TTGCGGGATTGAGGAGGGAC
  • BKK08310 ([gene|329E4DF9EF1545AABE485B9BBCD22F09ABF019A1|lnrL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCGTCTCACTCCTTTT, downstream forward: _UP4_TTGCGGGATTGAGGAGGGAC

    • References

  • 10092453,26647299,27766092,28461449